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以甘蓝Brassica oleracea L.感病品种“庆丰”为材料,通过叶面喷施冠菌素(coronatine,COR)和人工接种黑腐病菌——野油菜黄单胞菌野油菜致病变种(Xanthomonas campestris pv.campestris)的方法,研究了COR诱导甘蓝幼苗抗黑腐病的效果及其生理生化机制。结果表明,COR在质量浓度为0.01、0.1和1.0 mg/L下均具有诱导甘蓝幼苗抗黑腐病的效果,其中1.0 mg/L处理的病情指数为38.2,诱抗率为43.7%,诱导抗病效果最好。与对照相比,随着COR质量浓度的提高,叶片中过氧化氢酶(CAT)活性和超氧阴离子(O2-)产生的速率明显增加,而过氧化物酶(POD)的活性增加不明显,超氧化物歧化酶(SOD)活性和丙二醛(MDA)的含量明显下降。表明COR具有诱导甘蓝幼苗抗黑腐病的作用,而这种作用可能与COR能调控甘蓝幼苗中抗氧化酶活性、提高O2-产生速率以及降低MDA的含量相关。
In this study, Brassica oleracea L., a susceptible cultivar “Qingfeng”, was sprayed with coronatine (COR) through foliar spray and inoculated with black rot fungus, Xanthomonas campestris, (Xanthomonas campestris pv. Campestris), the effect of COR on cabbage seedling resistance to black rot and its physiological and biochemical mechanisms were studied. The results showed that COR had the effect of inducing the resistance to black rot of cabbage seedlings under the concentrations of 0.01, 0.1 and 1.0 mg / L, of which 1.0 mg / L treatment induced the disease index 38.2 and the induction rate 43.7% The best disease. Compared with the control, the CAT activity and the rate of superoxide anion (O2-) production in leaves increased significantly with the increase of COR concentration, while the increase of peroxidase (POD) activity was not obvious , Superoxide dismutase (SOD) activity and malondialdehyde (MDA) content decreased significantly. The results showed that COR had the effect of inducing the resistance of cabbage seedlings to black rot, and this effect may be related to the regulation of COR activity of antioxidant enzymes in cabbage seedlings, increasing the O2-producing rate and decreasing the content of MDA.