目的　探讨不同植瘤方式建立兔VX2 肝癌模型的成功率 ,并分析该肿瘤的生长特性。方法　 60只新西兰白兔随机分 3组 ,每组 2 0只。将VX2 瘤细胞 (5× 10 7个 )经肝动脉或经肝包膜分别接种于 2组兔的肝左叶 ,第 3组经肝包膜植入瘤组织块 (约含 10 6 ～ 10 8个瘤细胞 )建立肝癌模型。观察 :①不同组植瘤的成活率。②肿瘤 7d、10d、14d、17d、2 1d时的体积 (B超测 ) ,并计算肿瘤生长率。③大体及镜下 (光镜和电镜 )瘤组织形态特征。④VX2 移植性肝癌的DSA影像特征。结果　 3组植瘤成活率分别为 7/ 2 0、10 / 2 0、19/ 2 0 ,第 3组瘤块植瘤成活率最高 (Ρ <0 .0 5 ) ,瘤体呈指数性生长 ,组织病理及电镜表明该瘤在肝组织中浸润式生长 ,其性状与VX2 鳞状细胞癌特征相似。DSA影像示该移植性肝癌具有丰富的血供。结论　瘤块种植是建立兔肝癌模型的首选方式 ,该模型是肝癌的基础及临床的理想动物模型 Objective To investigate the success rate of establishing a rabbit VX2 liver cancer model with different tumor patterns and to analyze the growth characteristics of the tumor. Methods Sixty New Zealand white rabbits were randomly divided into 3 groups (20 in each group). The VX2 tumor cells (5 × 10 7) were inoculated into the left hepatic lobe of the rabbits in each group via the hepatic artery or pericardium, and the third group was implanted into the tumor mass (about 106 ~ 108 Tumor cells) to establish a liver cancer model. Observation: ① different groups of tumor survival rate. ② The tumor volume (B ultrasound) at 7d, 10d, 14d, 17d, 21d and the tumor growth rate were calculated. ③ General and microscopic (light and electron microscopy) tumor morphological features. ④VX2 transplanted liver cancer DSA image features. Results The survival rate of tumor in 3 groups was 7/20, 10/20/20 and 19/20 respectively. The survival rate of the tumor in the third group was the highest (P <0.05), the growth of the tumor was exponential, Histopathology and electron microscopy showed that the tumor grew infiltratively in liver tissue with similar characteristics to VX2 squamous cell carcinoma. DSA images show that the transplanted liver cancer has a rich blood supply. Conclusion Nodules are the first choice to establish rabbit liver cancer model. The model is the basis of liver cancer and the ideal clinical animal model