论文部分内容阅读
目的:利用氢化物发生-原子荧光光谱法,在L-半胱氨酸存在下,测定食品中的硒。方法:样品经酸加热消化后,用2%盐酸溶液做载流,原子荧光分光光度计进行测定。结果:方法的测定范围为10.0μg/L~50.0μg/L,相对标准偏差为0.70%~1.90%,回收率为93.0%~101.0%,检出限为0.051μg/L。结论:由于L-半胱氨酸的存在,改善了氢化物发生条件,增敏光谱测定信号,降低了溶液的酸度,金属离子的干扰显著地得到了抑制,适用于食品中硒的测定。
OBJECTIVE: To determine selenium in food by hydride generation-atomic fluorescence spectrometry in the presence of L-cysteine. Methods: Samples were acid digested with 2% hydrochloric acid solution to carry current, atomic fluorescence spectrophotometer for determination. Results: The determination range was 10.0μg / L ~ 50.0μg / L, the relative standard deviation was 0.70% ~ 1.90%, the recovery was 93.0% ~ 101.0%, and the detection limit was 0.051μg / L. Conclusion: Because of the presence of L-cysteine, the conditions of hydride generation are improved, the signal of sensitization is detected by sensitization spectroscopy, and the acidity of the solution is reduced. The interference of metal ions is significantly suppressed, which is suitable for the determination of selenium in food.