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为探讨丙二醛对外周血单核细胞是否具有趋化作用，本实验采用改良的Ｂｏｙｄｅｎ 小室微孔滤膜法进行单核细胞的趋化试验。用Ｋｉｋｕｇａｗａ 报道的方法制备丙二醛。结果发现，低、中、高三种浓度（０ ．０５ ｍｍｏｌＬ、０ ．５ ｍｍｏｌＬ和１ ｍｍｏｌＬ） 的丙二醛所诱导的单核细胞平均移动距离分别为８５．３７ ±１０ ．４４ μｍ 、１０９．０３ ±７．８８ μｍ 及１２２ ．６７ ±６．２５ μｍ ， 明显大于随机移动组（６９ ．８８ ±８ ．１９ μｍ）。方差分析表明，低浓度丙二醛组与随机移动组相比，差异有显著性意义（Ｐ＜０ ．０５） ，而中浓度和高浓度丙二醛组与随机移动组相比，差异有极显著性意义（Ｐ＜０．００１）。而且，丙二醛所诱导的单核细胞移动距离与其浓度呈剂量依赖关系。化学促动组单核细胞的平均移动距离（１１０．７２ ±７．３２ μｍ）亦明显大于随机移动组（ Ｐ＜ ０．００１） 。由此可见，丙二醛对外周血单核细胞有明显的趋化作用和化学促动作用。此结果提示，在动脉粥样硬化早期，丙二醛在动脉内膜中单核巨噬细胞的募集过程中起一定作用。
In order to investigate whether MDA has chemotaxis to peripheral blood mononuclear cells, a modified Boyden chamber microporous membrane method was used to test the chemotaxis of monocytes. Malondialdehyde was prepared by the method reported by Kikugawa. The results showed that the mean moving distance of mononuclear cells induced by malondialdehyde at low, medium and high concentrations (0 .05 mmolL, 0.5 mmolL and 1 mmolL) were 85.37 ± 10 . 44 μm, 109.03 ± 7.88 μm and 122. 67 ± 6.25 μm, which was significantly higher than that of the random movement group (69.88 ± 8.19 μm). Analysis of variance showed that there was significant difference (P <0. 05) between the low concentration of MDA group and the random shift group, while there was a significant difference between the medium and high concentration of MDA Significant significance (P <0.001). Moreover, the distance traveled by the malondialdehyde-induced monocytes in a dose-dependent manner. The average mobility of monocytes in the chemical-induced group (110.72 ± 7.32 μm) was also significantly greater than that in the randomized group (P <0.001). Thus, malondialdehyde on peripheral blood mononuclear cells have obvious chemotactic and chemical activation. This result suggests that at the early stage of atherosclerosis, MDA plays a role in the recruitment of mononuclear macrophages in the arterial intima.