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苯并(a)芘广泛存在于煤焦油、炼油等工业污水中,多次使用的高温植物油、烧焦的食物都可能含有苯并(a)芘。我国目前对食用植物油类产品中苯并(a)芘的限量标准是<10μg/kg。检测食品中苯并(a)芘的方法中使用较多的是高效液相色谱法、气相色谱-质谱联用法等方法,但检测过程样品的前处理复杂、检测耗时长、检测效率低,尚不能满足实时、现场检测的要求。本文使用纳米金修饰的多孔材料作为SERS基底,利用SERS对食用油中的苯并(a)芘的含量进行快速检测。苯并(a)芘在0~200μg/L范围内与SERS的信号值呈线性关系,线性方程为y=134.98x-571.77,R2=0.9942;在10μg/kg含量水平下,样品回收率为93.2~125.7%,相对标准偏差为10~18%,方法检出限为5.6μg/L。SERS可使用便携式拉曼光谱仪进行现场检测,测试时间短(一般为数十秒至一两分钟)。在检测灵敏度满足要求的情况下,SERS方法为快速筛查食用油中苯并(a)芘含量提供了一种新的选择。
Benzo (a) pyrene is widely present in coal tar, oil refining and other industrial effluents. High temperature vegetable oil and charred food which are used repeatedly may contain benzo (a) pyrene. At present, the limit of benzo (a) pyrene in edible vegetable oil products in our country is <10μg / kg. Detection of benzo (a) pyrene in food using more methods are high performance liquid chromatography, gas chromatography - mass spectrometry and other methods, but the detection process sample pretreatment complex, long detection time, low detection efficiency, yet Can not meet the real-time, on-site testing requirements. In this paper, nano-gold porous material modified as a SERS substrate, the use of SERS edible oil benzo (a) pyrene content for rapid detection. The linear relationship was obtained between benzo (a) pyrene and SERS in the range of 0 ~ 200μg / L with the linear equation of y = 134.98x-571.77 and R2 = 0.9942. The recovery of the sample was 93.2 at the concentration of 10μg / kg ~ 125.7%, the relative standard deviation is 10 ~ 18%, and the detection limit is 5.6μg / L. SERS can be detected on-site using a portable Raman spectrometer for short test times (typically tens of seconds to a minute or two). The SERS method provides a new option for the rapid screening of benzo (a) pyrene in edible oils with satisfactory sensitivity.