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目的:探讨Osterix(Osx)对乳腺癌细胞MDA-MB-231迁移和侵袭能力的影响及其潜在机制。方法:采用荧光实时定量PCR(Real-time PCR)及Western blot技术检测Osx过表达及敲低稳转细胞(231-OE6、231-OEC、231-KD2、231-KDC)中赖氨酰氧化酶(lysyl oxidase,LOX)的表达水平;转染LOX si RNA及其对照至Osx过表达稳转细胞(231-OE6)中,Transwell检测细胞的迁移及侵袭能力;同时转染LOX过表达质粒及其对照至Osx敲低稳转细胞(231-KD2)中,Transwell检测细胞的迁移及侵袭能力。结果:与各自对照相比,LOX在231-OE6中表达显著升高,在231-KD2中表达显著降低(P<0.05);降低LOX的表达能够显著削弱231-OE6细胞的迁移和侵袭能力,增加LOX的表达能够显著提高231-KD2细胞的迁移和侵袭能力,差异有统计学意义(P<0.05)。结论:在乳腺癌细胞中Osx能够上调LOX的表达,且Osx通过上调LOX的表达而促进乳腺癌细胞的迁移和侵袭。
Objective: To investigate the effect of Osterix (Osx) on migration and invasion of breast cancer cell MDA-MB-231 and its potential mechanism. Methods: Real-time PCR and Western blot were used to detect the expression of lysyl oxidase in Osx overexpression and knockdown cells (231-OE6, 231-OEC, 231-KD2, 231-KDC) (LOX). Transfection of LOX si RNA and its control to Osx overexpression stable transfection cells (231-OE6), Transwell assay of cell migration and invasion ability; simultaneous transfection LOX overexpression plasmid and its Transfection was used to detect the migration and invasion of cells in control knock-down Osx knockdown cells (231-KD2). Results: The expression of LOX was significantly increased in 231-OE6 and significantly decreased in 231-KD2 (P <0.05). LOX expression was significantly attenuated in 231-OE6 cells, Increasing the expression of LOX could significantly increase the migration and invasion ability of 231-KD2 cells, the difference was statistically significant (P <0.05). CONCLUSIONS: Osx upregulates LOX expression in breast cancer cells and Osx promotes the migration and invasion of breast cancer cells by up-regulating LOX expression.