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目的研究新疆结核分枝杆菌Spoligotyping基因分型,初步了解其基因型多态性状况及主要流行株。方法在新疆维吾尔自治区胸科医院收集一个连续时间段的结核分枝杆菌临床分离菌株,采用比例法检测进行耐药性检测、间隔寡核苷酸分型(Spoligotyp ing)方法进行分型研究。基因聚类分析采用B ioNum erics 5.0数据库软件,统计学分析采用χ2检验,P<0.05为差异有统计学意义。结果共收集到结核分枝杆菌临床分离菌株175株,其中对利福平、异烟肼、链霉素和乙胺丁醇全敏感115株,耐药60株(包括单耐药31株和耐多药29株)。经Spoligotyp ing分型,这些菌株可分为4个基因群49种基因型,最大的1个基因群为北京家族,占68.57%(120/175)。北京家族中,敏感菌株63.87%(76/119),耐药菌株占36.13%(43/119);非北京家族中,敏感菌株68.52%(37/54),耐药菌株占31.48%(17/54),北京家族与非北京家族的耐药率之间差异无统计学意义(P=0.551)。结论新疆结核分枝杆菌临床菌株存在明显的基因多态性,主要流行菌株为北京基因型。北京基因型与耐药性无明显相关性。
Objective To study the genotyping of Mycobacterium tuberculosis Spoligotyping in Xinjiang and to understand its genotype polymorphism and its main epidemic strains. Methods A clinical isolates of Mycobacterium tuberculosis were collected in the Chest Hospital of Xinjiang Uygur Autonomous Region for testing the drug resistance and genotyping by Spoligotyping method. Genetic clustering analysis using B ioNum erics 5.0 database software, statistical analysis using χ2 test, P <0.05 for the difference was statistically significant. Results A total of 175 clinical isolates of Mycobacterium tuberculosis were collected, of which 115 were susceptible to rifampicin, isoniazid, streptomycin and ethambutol, 60 were resistant (including 31 single drug resistant and resistant 29 multi-drug). According to Spoligotyp ing, these strains can be divided into 49 genotypes of 4 gene groups, and the largest one is Beijing family, accounting for 68.57% (120/175). Among Beijing families, 63.87% (76/119) were susceptible strains and 36.13% (43/119) were resistant strains; in non-Beijing families, 68.52% (37/54) were sensitive strains and 31.48% were resistant strains (17 / 54). There was no significant difference in resistance rates between Beijing and non-Beijing families (P = 0.551). Conclusion There are obvious gene polymorphisms in clinical strains of Mycobacterium tuberculosis in Xinjiang. The main epidemic strains are Beijing genotypes. Beijing genotype and drug resistance no significant correlation.