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目的分析吉林省2013年麻疹病毒流行株的分子流行病学特征。方法对50份麻疹病毒逆转录-聚合酶链反应阳性产物进行麻疹病毒核苷酸序列测定,采用分子进化遗传分析4.0软件构建基因亲缘性关系树。采用地理信息系统软件分析麻疹病毒传播的地理分布特点。结果 50株H1a基因亚型麻疹病毒在基因树上形成3个相对独立的传播链(Clade 1、Clade 2、Clade 3)。传播链Clade 1、2、3分别分布在10个、5个、9个县(区、市,下同);东部高发区域以Clade2传播链为主,中部高发区域以3种传播链混合存在,西部高发区域有可能存在传播链Clade3。结论3条传播链均分别或共同分布在多个县,一个县可有一个或多个传播链导致麻疹流行。
Objective To analyze the molecular epidemiological characteristics of measles virus in Jilin province in 2013. Methods Fifty measles virus reverse transcriptase - polymerase chain reaction (PCR) positive products were sequenced and the phylogenetic tree was constructed by using molecular evolution genetic analysis software 4.0. Using Geographic Information System software to analyze the geographical distribution of measles virus. Results Fifty H1a subtype measles viruses formed three relatively independent transmission chains (Clade 1, Clade 2 and Clade 3) in the gene tree. The distribution chain Clade 1, 2, 3 are distributed in 10, 5 and 9 counties (districts, cities, the same below); the eastern part of the high incidence of the distribution chain Clade2 mainly in the central high incidence of three kinds of transmission chain mixed exist, There may be a transmission chain Clade3 in the western high incidence area. Conclusion The three transmission chains are distributed separately or co-located in multiple counties. One county may have one or more transmission chains leading to measles epidemic.