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采用ＨＧＶＮＳ５特异的２对引物，对两个香港株和一个广东株ＨＧＶＲＮＡ进行逆转录套式ＰＣＲ扩增，ＰＣＲ产物克隆入ｐＵＣ１９，重组质粒转化ＤＨ５α和ＪＭ１０９菌株。ＰＣＲ和酶切法鉴定阳性克隆，双脱氧链末端终止法测定核苷酸序列并进行同源性分析。结果发现核苷酸变异呈散在分布，三株间核苷酸和氨基酸序列同源性分别为９３．３％～９４％及９７％～９９．２％，与已报道的中国株（ＣＮ）相比，则同源性分别为９０％～９１．２％和９４％～９６．３％，与美国株（ＰＮＦ２１６１及Ｒ１０２９１）相比，为８７．１％～８９．５％和９５．２％～９７％，而与西非株（ＧＢＶＣ）相比，则达９１．４％～９３．８％和９７％～９７．９％。提示ＨＧＶＮＳ５区核苷酸和氨基酸序列相对保守，不同ＨＧＶ株存在一定的地区差异。
Two HGVNS5-specific primers were used to amplify the HGVRNA of two Hong Kong strains and one Guangdong strain. The PCR products were cloned into pUC19 and the recombinant plasmids were transformed into DH5α and JM109 strains. The positive clones were identified by PCR and restriction enzyme digestion. The nucleotide sequences were determined by dideoxy chain termination and analyzed by homology. The results showed that the nucleotide variation was scattered. The nucleotide and amino acid sequence identities of the three strains were 93.3% ~ 94% and 97% ~ 99.2%, respectively. Compared with the reported CN strain The homology was between 90% and 91.2% and 94% and 96.3% respectively, which was 87.1% -89.5% and 95.2% compared with that of the American strain (PNF2161 and R10291) ~ 97% compared with that of West Africa strain (GBV-C), reaching 91.4% ~ 93.8% and 97% ~ 97.9%. Suggesting that the nucleotide and amino acid sequences of HGVNS5 region are relatively conservative, and there are some regional differences in different HGV strains.