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目的探讨S-腺苷酰-L-甲硫氨酸(S-adenosyl-L-methionine,SAMe)对肝癌细胞HepG2增殖、迁移及癌基因C-myc表达的影响,为深入研究SAMe在肝癌治疗中的作用奠定基础。方法体外培养HepG2细胞,MTT法检测不同浓度SAMe作用不同时间对HepG2细胞增殖的影响;细胞划痕法检测最适浓度SAMe对HepG2细胞迁移能力的影响;RT-PCR、免疫细胞化学法及Westernblot法检测SAMe对HepG2细胞癌基因C-myc转录、蛋白定位及表达的影响。结果终浓度为15.0μmol/L的SAMe处理细胞5d,细胞抑制率达54.6%,且抑制作用呈时间与剂量依赖性;经15.0μmol/LSAMe处理的细胞迁移能力明显下降,愈合速率为对照组的76.78%;细胞中癌基因C-myc的转录和蛋白表达均明显下降,蛋白定位无明显变化。结论 SAMe可通过降低C-myc的表达,抑制HepG2细胞的增殖和迁移,为肝癌治疗性药物的研究提供了一个新的方向。
Objective To investigate the effects of S-adenosyl-L-methionine (SAMe) on the proliferation, migration and oncogene C-myc expression in HepG2 hepatocellular carcinoma cells. To study the effect of SAMe on hepatocellular carcinoma The role of foundation. Methods HepG2 cells were cultured in vitro. The effect of different concentrations of SAMe on the proliferation of HepG2 cells was detected by MTT assay. The effect of SAMe on the migration ability of HepG2 cells was determined by cell scratch assay. The effects of SAMe on the migration of HepG2 cells were detected by RT-PCR, immunocytochemistry and Western blot To detect the effect of SAMe on C-myc transcription, localization and expression of oncogene in HepG2 cells. Results After treated with 15.0μmol / L SAMe for 5 days, the cell inhibitory rate reached 54.6% and the effect was dose and time dependent. The migration ability of SAMe cells treated with 15.0μmol / L SAMe decreased significantly, and the healing rate of control group 76.78%. The transcription and protein expression of C-myc in the cell were significantly decreased, but the protein localization did not change significantly. Conclusions SAMe can inhibit the proliferation and migration of HepG2 cells by decreasing the expression of C-myc and provide a new direction for the study of therapeutic drugs for hepatocellular carcinoma.