论文部分内容阅读
目的研究经典复方四君子汤与FU合用诱导胃癌细胞凋亡的作用机理。方法用四君子汤含药血清和稀释的FU溶液作用于人胃癌细胞SGC-7901细胞株,免疫组化法检测凋亡相关基因p53、Bcl-2和Bax的表达。结果四君子汤含药血清与FU合用能明显下调Bcl-2的表达,上调Bax的表达,对p53表达没有显著性影响。结论四君子汤与FU合用诱导人胃癌细胞SGC-7901凋亡的作用机理可能与下调Bcl-2的表达,上调Bax的表达有关,而非依赖于p53途径。该实验为临床中西医结合治疗胃癌提供一定的理论依据。
Objective To study the mechanism of classical compound Sijunzi Decoction combined with FU in inducing gastric cancer cell apoptosis. Methods SGC-7901 cells were treated with Sijunzi decoction serum and diluted FU solution. The expression of p53, Bcl-2 and Bax were detected by immunohistochemistry. Results Sijunzi decoction combined with FU could obviously down-regulate the expression of Bcl-2 and up-regulate the expression of Bax, but had no significant effect on the expression of p53. Conclusion Sijunzi decoction can induce the apoptosis of human gastric cancer cell SGC-7901 in combination with FU, which may be related to down-regulating the expression of Bcl-2 and up-regulating the expression of Bax, but not to the p53 pathway. The experiment for clinical clinical treatment of gastric cancer with a certain theoretical basis.