长期以来国内外研究疼痛与镇痛机理多应用的是急性痛的动物模型,但在临床上更多遇到的是持续性慢痛,因此要求制造持久的伤害性刺激的慢痛模型才接近临床实际。给动物注射formalin试验最早是在1977年,由Dubuisson和Dennis在鼠和猫身上进行研究的,浓度为5%,注射量50μl,注射后30～60min期间观察其反应,按出现时间先后有一个早反应(earlg response)和一个迟反应(late response),1984年 Long-term domestic and international research on pain and analgesic mechanism of multiple application of the animal model of acute pain, but more clinically encountered persistent slow pain, and therefore requires the manufacture of long-lasting nociceptive slow pain model is close to clinical actual. The injection of formalin into animals was first studied in 1977 by Dubuisson and Dennis in rats and cats at a concentration of 5% with an injection volume of 50 μl. The response was observed during 30-60 min after injection, with an early onset Earlg response and a late response, 1984