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本研究旨在探讨犬急性胰腺炎(AP)时血清白介素6 (IL-6)水平的变化及其与胰腺组织的损伤程度之间的关系。材料与方法1.动物模型制备选用成都军区总医院实验动物中心提供的本地健康成年杂种犬30条,雌雄不拘,体重9.5～14.3kg(11.7±12.lkg)。经随机分组后,采用自身胆汁在不同压力、持续不同时间灌注胰管,制备成急性水肿性胰腺炎(AEP)11例,急性出血坏死性胰腺炎(AHNP)12例,对照组7例。2.标本采集及样本制备分别于发病前、发病后3、12、24及48小时在无菌条件下经股静脉采血,标本
The purpose of this study was to investigate the changes of serum interleukin 6 (IL-6) level and its relationship with the degree of pancreatic tissue damage in acute pancreatitis (AP). MATERIALS AND METHODS 1. Preparation of animal model Thirty healthy adult mongrel dogs were provided by Experimental Animal Center of Chengdu Military Region General Hospital with a weight of 9.5 ~ 14.3kg (11.7 ± 12.lkg). After randomization, 11 cases of acute edematous pancreatitis (AEP), 12 cases of acute hemorrhagic necrotizing pancreatitis (AHNP) and 7 cases of control group were prepared by perfused pancreatic duct with different bile pressure at different times. 2. Specimen collection and sample preparation Before onset, 3,12,24 and 48 hours after onset of aseptic conditions through the femoral vein blood samples