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目的:探讨胃癌患者外周血T细胞亚群的变化及其对评估胃癌患者机体免疫状态的临床意义。方法:流式细胞术分析40例术前胃癌患者及30名健康人群(对照组)外周血中T细胞亚群(CD3+、CD4+、CD8+T细胞、CD4+/CD8+比值),组间比较采用t检验,检验水准a=0.05。结果:研究组胃癌患者外周血CD3+、CD4+T细胞、CD4+/CD8+比值明显低于健康人群对照组,差异有统计学意义(P值均<0.05),而两组间CD8+T细胞的差异无统计学意义(P>0.05);肿瘤侵及浆膜组CD3+、CD4+T细胞、CD4+/CD8+比值明显低于未侵及浆膜组,差异有统计学意义(P值均<0.05);肿瘤有淋巴结转移组CD3+、CD4+T细胞、CD4+/CD8+比值明显低于肿瘤无淋巴结转移组,差异有统计学意义(P值均<0.05);肿瘤临床分期TNMⅢ、Ⅳ期组CD3+、CD4+T细胞、CD4+/CD8+比值明显低于TNMⅠ、Ⅱ期组,差异有统计学意义(P值均<0.05);肿瘤高中分化组和低分化组间CD3+、CD4+T细胞、CD4+/CD8+比值的差异无统计学意义(P值均>0.05),各不同组间外周血CD8+T细胞的差异无统计学意义(P值均>0.05)。结论:胃癌患者术前外周血T细胞亚群对评估胃癌患者机体免疫状态有一定的临床意义,也为肿瘤的免疫增强治疗提供了理论依据。
Objective: To investigate the changes of T cell subsets in peripheral blood of patients with gastric cancer and its clinical significance in assessing the immune status of patients with gastric cancer. Methods: T cell subsets (CD3 +, CD4 +, CD8 + T cells, CD4 + / CD8 + ratio) in 40 patients with preoperative gastric cancer and 30 healthy people (control group) were analyzed by flow cytometry. Inspection, inspection level a = 0.05. Results: The ratio of CD3 +, CD4 + T cells and CD4 + / CD8 + in peripheral blood of gastric cancer patients in study group was significantly lower than that in healthy controls (P <0.05), while the difference of CD8 + T cells (P> 0.05). The ratio of CD3 +, CD4 + T cells and CD4 + / CD8 + in invasive tumor and serosal invasion group was significantly lower than that in noninvasive and serosal invasion group (P <0.05). The ratio of CD3 +, CD4 + T cells and CD4 + / CD8 + in lymph node metastasis group was significantly lower than that in non-lymph node metastasis group (all P <0.05) T cells and CD4 + / CD8 + ratio were significantly lower than those in TNMⅠ and Ⅱ groups (all P <0.05). The ratio of CD3 +, CD4 + T cells, CD4 + / CD8 + There was no significant difference between the two groups (P> 0.05). There was no significant difference in peripheral blood CD8 + T cells among different groups (P> 0.05). Conclusion: Preoperative T-cell subsets of peripheral blood in patients with gastric cancer have certain clinical significance for assessing the immune status of patients with gastric cancer, and also provide a theoretical basis for the immune enhancement of tumors.