目的:探讨7-酮基胆固醇(7-KC)作用小鼠巨噬细胞J774A.1后,NF-κB的活化与细胞凋亡的关系。方法:细胞经7-KC处理后,通过形态学观察,MTT检测法,DNA电泳,流式细胞术检测细胞凋亡水平。用Westernblot和免疫组织化学方法检测NF-κB活化情况,采用二硫代氨基甲酸吡咯烷(PDTC)抑制实验检测NF-κB活化对7-KC诱导的细胞凋亡的影响。结果:7-KC能抑制小鼠巨噬细胞的生长并促进细胞凋亡,同时能诱导小鼠巨噬细胞NF-κB的活化,且NF-κB特异性抑制剂PDTC能显著抑制7-KC诱导的细胞凋亡。结论:7-KC能通过活化NF-κB途径诱导巨噬细胞发生凋亡。 Objective: To investigate the relationship between the activation of NF-κB and the apoptosis of J774A.1 induced by 7-ketochol (7-KC) in mouse macrophages. Methods: After the cells were treated with 7-KC, the cell apoptosis was detected by morphological observation, MTT assay, DNA electrophoresis and flow cytometry. The activation of NF-κB was detected by Western blot and immunohistochemistry, and the effect of NF-κB activation on 7-KC-induced apoptosis was detected by inhibition of dithiocarbamate pyrrolidine (PDTC). Results: 7-KC can inhibit the growth of mouse macrophages and promote apoptosis, meanwhile, it can induce the activation of NF-κB in mouse macrophages, and PDTC can significantly inhibit the induction of 7-KC Apoptosis. Conclusion: 7-KC can induce apoptosis of macrophages by activating NF-κB pathway.