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目的:建立RP-HPLC-DAD方法,同时测定复方石韦胶囊中苦参酮、槐属二氢黄酮G、异黄腐醇、三叶豆紫檀苷4个黄酮含量。方法:采用C_(18)色谱柱(250 mm×4.6 mm,5μm),以甲醇-水为流动相梯度洗脱,流速1mL·min~(-1),检测波长300 nm。结果:苦参酮、槐属二氢黄酮G、异黄腐醇、三叶豆紫檀苷线性范围分别为0.34~6.84μg(r=0.999 3)、0.16~3.27μg(r=0.999 5)、0.04~0.85μg(r=0.999 1)、0.19~3.70μg(r=0.999 8);精密度、重复性良好,RSD均小于2.0%;在室温条件下供试溶液12 h内稳定;平均加样回收率(n=6)在99.2%~101.5%(RSD≤2.2%)。3批样品中上述4个成分平均含量依次为94.84~98.85、44.73~47.79、11.16~13.26、54.17~58.74μg·粒~(-1)。结论:所建立的分析方法可用于复方石韦胶囊中4个黄酮成分的含量测定。
OBJECTIVE: To establish a RP-HPLC-DAD method for the simultaneous determination of 4 flavonoids in Fufang Shwewei Capsules, including the contents of matrine, genistein, isoflavone, and trifolin. Methods: The mobile phase was eluted with C 18 column (250 mm × 4.6 mm, 5 μm) with a flow rate of 1 mL · min -1. The detection wavelength was 300 nm. Results: The linear ranges of matrine, geniposide G, isoflavone, and trifolinomycin were 0.34-6.84μg (r = 0.999 3), 0.16-3.77μg (r = 0.999 5), 0.04 ~ 0.85μg (r = 0.999 1), 0.19 ~ 3.70μg (r = 0.999 8). The precision and reproducibility were good with RSD less than 2.0%. The test solution was stable within 12 h at room temperature. Rates (n = 6) ranged from 99.2% to 101.5% (RSD ≤ 2.2%). The average contents of the above four components in three batches of samples were 94.84 to 98.85, 44.73 to 47.79, 11.16 to 13.26 and 54.17 to 58.74 μg · kg -1, respectively. Conclusion: The established analytical method can be used to determine the content of four flavonoids in Fufang Shiwei capsule.