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从化学诱发的肿瘤或转化的细胞中分析瘤基因及其活化有助于从分子、基因水平阐明肿瘤形成的机理。以往的瘤基因分析方法大多数采用以ＮＩＨ3Ｔ3为受体细胞的ＤＮＡ转染分析 ,但该方法主要根据ＤＮＡ转染后细胞形态改变 ,即折光性加强和停泊非依赖性生长来检测活化瘤基因 ,因此
Analysis of tumor genes and their activation from chemically-induced tumors or transformed cells helps clarify the mechanism of tumor formation at the molecular and genetic level. Most of the past tumor gene analysis methods using NIH3T3 as the recipient cell DNA transfection analysis, but the method is mainly based on DNA transfection cell morphology changes, that is, enhanced refractive and anchorage-independent growth to detect activated tumor genes, therefore