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目的观察姜黄素对支气管肺发育不良(BPD)大鼠模型的干预作用,探讨姜黄素对BPD的可能保护机制。方法足月新生SD大鼠108只随机分为3组:正常对照组;600mL/L O2处理组;姜黄素干预组。于实验的第4、7、14d每组随机处死8只,HE染色观察肺组织病理结构改变;ELISA法检测血清和肺组织匀浆白介素(IL)-6、IL-10的表达;免疫组织化学法检测肺组织胰岛素样生长因子-Ⅰ(IGF-Ⅰ)蛋白的表达。结果肺组织形态学改变显示600mL/L O2处理组随着氧气暴露时间的延长,出现肺发育受阻表现,部分肺泡间隔变薄,肺泡大而简单化,而姜黄素干预组病理改变较600mL/L O2处理组改善;与正常对照组比较,600mL/L O2处理组和姜黄素干预组血清和肺组织匀浆IL-6和IL-10的表达均增高(P<0.05);姜黄素干预组IL-6的表达较600mL/L O2处理组下降,而IL-10的表达则上升(P<0.05);第4、7d时IGF-Ⅰ的表达在正常对照组中高于600mL/L O2处理组,第14d时低于600mL/L O2处理组,而姜黄素干预能增加BPD肺组织IGF-Ⅰ在第4、7d的表达(P<0.05),降低其在第14d的表达(P<0.05)。结论姜黄素可通过调控IL-6、IL-10和IGF-Ⅰ的合理表达部分逆转高氧引发的肺发育阻滞。
Objective To observe the intervention of curcumin on rat model of bronchopulmonary dysplasia (BPD) and explore the possible protective mechanism of curcumin on BPD. Methods 108 full-term newborn SD rats were randomly divided into three groups: normal control group, 600mL / L O2 treatment group and curcumin intervention group. Eight rabbits were sacrificed on the 4th, 7th, and 14th day after the experiment. The pathological changes of the lung tissues were observed by HE staining. The expressions of interleukin (IL) -6 and IL-10 in serum and lung tissue were detected by ELISA. Method to detect the expression of insulin-like growth factor-Ⅰ (IGF-Ⅰ) protein in lung tissue. Results The morphological changes of lung tissue showed that the development of lung development was obstructed with the prolonged oxygen exposure time in 600mL / L O2 treatment group, some of the alveolar septum became thinner and the alveoli were larger and more simple. The pathological changes of curcumin intervention group were more than 600mL / L (P <0.05). Compared with normal control group, the expression of IL-6 and IL-10 in serum and lung homogenate of 600 mL / L O2-treated group and curcumin-treated group increased (P <0.05). At the 4th and 7th day, the expression of IGF-Ⅰ was higher in the normal control group than in the 600mL / L O2 treatment group (P <0.05) On the 14th day, the level of IGF-Ⅰ in the lung tissue of BPD group was lower than that of the control group (600mL / L O2). Curcumin intervention could increase the expression of IGF-Ⅰ in lung tissue of BPD group on the 4th and 7th day (P <0.05) Conclusion Curcumin can partly reverse the hyperoxia-induced lung development block by regulating the rational expression of IL-6, IL-10 and IGF-Ⅰ.