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目的:研究肿瘤坏死因子α(TNF-α)在实验性自身免疫性脑脊髓炎(EAE)发病中的作用,观察中药复方益肾达络饮干预EAE的疗效,探讨其治疗EAE的作用机制。方法:选用雌性SJL/J小鼠95只,随机分为正常组、泼尼松3.9 mg·kg-1组、益肾达络饮20 g·kg-1组、益肾达络饮20 g·kg-1加泼尼松3.9 mg·kg-1组,从开始造模后7 d开始ig给药,连续15 d,从急性期、复发期采用免疫蛋白印迹法、免疫组化法测定EAE小鼠中枢神经组织中TNF-α蛋白表达的变化。结果:益肾达络饮能降低EAE的复发率(P<0.05),改善EAE模型小鼠神经功能评分(P<0.05)。与模型组相比,泼尼松3.9 mg·kg-1组、益肾达络饮20 g·kg-1组、益肾达络饮20 g·kg-1加泼尼松3.9 mg·kg-1组中枢神经组织TNF-α表达水平明显降低(P<0.05)。结论:降低TNF-α的表达水平,有助于缓解EAE病损程度;益肾达络饮能降低EAE的复发率,有效改善EAE小鼠神经功能损伤;醋酸泼尼松和益肾达络饮调节TNF-α的表达水平可能是治疗EAE的机制之一。
Objective: To investigate the role of tumor necrosis factor-α (TNF-α) in the pathogenesis of experimental autoimmune encephalomyelitis (EAE) and to observe the therapeutic effect of Yishen Daluo Decoction on EAE and to explore its mechanism of action in treating EAE. Methods: 95 female SJL / J mice were randomly divided into normal group, prednisone 3.9 mg · kg-1 group, Yishen Daluo drinking 20 g · kg-1 group and Yishen Daluo drinking 20 g · kg-1 plus prednisone 3.9 mg · kg-1, and administered ig for 7 days starting from the 7th day after the start of modeling. Immunohistochemical method was used to detect EAE in acute phase and relapse phase. Changes of TNF-α protein expression in rat central nervous system. Results: Yishen Daluo drinking can reduce the recurrence rate of EAE (P <0.05), improve the neurological function score of EAE model mice (P <0.05). Compared with model group, prednisone 3.9 mg · kg-1 group, Yishen Daluo drinking 20 g · kg-1 group, Yishen Daluo drinking 20 g · kg-1 plus prednisone 3.9 mg · kg- The expression of TNF-α in central nervous system of 1 group was significantly lower (P <0.05). Conclusion: Reducing the expression of TNF-α may help to alleviate the damage of EAE. Yishen Daluo Drink can reduce the recurrence rate of EAE and effectively improve the neurological impairment of EAE mice. Prednisone acetate and Yishen Daruo Drink Regulating the expression level of TNF-α may be one of the mechanisms for treating EAE.