本文分别以APAAP桥联酶标技术、生物活性法及放免法测定了本科67例食管癌病人手术前、后及22例根治术后随访病人外周血自然杀伤(NK)细胞数量和血浆肿瘤坏死因子(TNF)、前列腺素E_2(PGE_2)水平,并探讨其临床意义。20例健康献血员为正常对照组。实验组病例均经严格选择,排除感染或创伤对实验结果的影响。研究结果发现术前食 In this paper, the number of natural killer (NK) cells and plasma tumor necrosis factor in peripheral blood of 67 patients with esophageal cancer before and after surgery and 22 patients after radical resection were measured by APAAP bridge enzyme-labeled technique, biological activity method and radioimmunoassay, respectively. (TNF), prostaglandin E 2 (PGE 2) levels, and its clinical significance. Twenty healthy blood donors were normal controls. All cases in the experimental group were rigorously selected to exclude the effect of infection or trauma on the experimental results. Findings before surgery