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为了解猪萨佩罗病毒(porcine sapelovirus,PSV)的流行及变异情况,采集2015-2016年四川地区12个县市34个猪场共计428份腹泻病料,采用QRT-PCR方法对PSV进行检测。结果显示:在428份病料中,共有114份为PSV阳性,阳性率为26.6%;所检测的34个猪场中,共有20个猪场显示PSV阳性,猪场阳性率为58.8%,表明PSV在四川地区普遍存在。此外,对来自不同县市共14份PSV阳性病料1B全基因进行PCR扩增,通过分子克隆,测序,再利用序列分析软件对四川部分地区猪萨佩罗病毒1B基因进行序列分析。结果表明,14条猪萨佩罗病毒1B基因核苷酸序列及推导出的氨基酸序列相似性分别为90.6%~100%和97.1%~100%。遗传进化分析显示,该研究获得的四川株与已知中国株分属于一个大的分支,说明我国猪萨佩罗病毒1B基因序列较为保守,没有发生大的基因变异。所有中国分离株与韩国株、德国株、英国株处在不同分支,又说明我国PSV的流行毒株可能存在独立进化。
In order to understand the prevalence and variation of porcine sapelovirus (PSV), 428 samples of diarrhea were collected from 34 pig farms in 12 counties and cities in Sichuan province during 2015-2016. PSV was detected by QRT-PCR . The results showed that 114 out of 428 samples were positive for PSV, with a positive rate of 26.6%. Of the 34 pig farms tested, 20 pig farms showed positive PSV, and the positive rate in pig farms was 58.8% PSV widespread in Sichuan. In addition, a total of 14 PSV-positive isolates from 14 counties in different counties were amplified by PCR. Sequence analysis of the 1B gene of pigs sapropel strain in Sichuan province was carried out by molecular cloning, sequencing and sequence analysis software. The results showed that the nucleotide and deduced amino acid sequences of the 1B sapropel virus 1B gene were 90.6% -100% and 97.1% -100%, respectively. Genetic analysis indicated that the Sichuan strain obtained from this study belonged to a large branch with the known Chinese strain, indicating that the gene sequence of 1B in sapropelaplase was relatively conservative and no major gene mutation occurred in China. All Chinese isolates and Korean strains, German strains, British strains in different branches, but also shows that the epidemic of PSV in our country may exist independent evolution.