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在“异养-胁迫”分段培养模式下分析原始小球藻(Chlorella protothecoides)的生物量及油脂含量。结果表明,在500ml摇瓶中,分段培养生物量达5.32g/L,略低于一步异养培养5.45 g/L,油脂含量达34.81%,是一步异养培养的2.26倍,藻渣中微藻多糖含量由9.57%提高到18.06%。在3L发酵罐中,一步异养培养模式下生物量为14.1 g/L,油脂含量为17.16%,微藻多糖含量为10.16%;而“异养-胁迫”分段培养模式下生物量13.20g/L,油脂含量40.15%,微藻多糖含量24.74%。本研究显示“异养-胁迫”分段培养模式是一种快速获得大量高油脂含量藻细胞的有效方法,可为规模化生产微藻生物柴油和进一步利用微藻奠定了技术基础。
The biomass and oil content of Chlorella protothecoides were analyzed in a “heterotrophic-stress” staged culture model. The results showed that in 500ml shake flask, the biomass of the segmented culture reached 5.32g / L, slightly lower than the one-step heterotrophic culture of 5.45g / L, the fat content reached 34.81%, which was 2.26 times of that of one-step heterotrophic culture Microalgae polysaccharide content increased from 9.57% to 18.06%. In the 3L fermenter, biomass was 14.1 g / L in one-step heterotrophic culture, the lipid content was 17.16% and the content of polysaccharide in microalgae was 10.16%, while the biomass in “heterotrophic-stress” 13.20g / L, oil content 40.15%, microalgae polysaccharide content 24.74%. This study shows that the “heterotrophic-stress” segmented culture mode is an effective method for rapidly obtaining large amount of algal cells with high oil content, which lays the technical foundation for the large-scale production of microalgal biodiesel and the further utilization of microalgae.