集落刺激因子是研究粒系祖细胞形成G、M集落过程中的重要物质,不同来源的集落刺激因子,都可以达到促使粒系祖细胞增殖、分化而形成集落。笔者观察到,从羊水中分离出的集落刺激因子,其分子量不同,对粒系祖细胞集落形成的速度有所差异。本文概述其原理、集落形成条件、制作分离方法以及活性鉴定等问题。 Colony-stimulating factor is an important material in the process of granulocyte-derived progenitor cells forming G, M colonies. Colony stimulating factors from different sources can all promote the proliferation and differentiation of granulocyte progenitor cells to form colonies. The author observed that the colony stimulating factor isolated from amniotic fluid, its molecular weight, the rate of granulocyte progenitor colony formation are different. This article outlines its principles, colony formation conditions, production and isolation methods, and identification of active ingredients.