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建立了高效液相色谱测定伪麻黄碱血药浓度的方法。血浆样品用乙醚-正己烷(1∶1V/V)提取,LiChrosorb 100CN为固定相,流动相为乙腈-甲醇-磷酸二氢钾-庚烷磺酸钠-重蒸水,检测波长为210 nm。线性范围为10.0～800.0 ng/ml,回收率>90%,日内、日间RSD<10%。12名男性受试者单剂量服用240 mg伪麻黄碱缓释片所得药代动力学参数为:T_(max)=8.58±2.47h;C_(max)=358.47±140.43mg/ml;t_(1/2)=10.27±3.04h;MRT=18.84±4.12h;AUC=7403.6±2166.6ng·h/ml。
A HPLC method for the determination of pseudoephedrine in plasma was established. Plasma samples were extracted with ether-n-hexane (1: 1 V / V). LiChrosorb 100CN was used as the stationary phase. The mobile phase consisted of acetonitrile-methanol-potassium dihydrogen phosphate-heptane sulfonate-water. The detection wavelength was 210 nm. The linear range was 10.0-800.0 ng / ml, the recovery rate was> 90%, and the RSD was <10%. Pharmacokinetic parameters of 12 male subjects taking 240 mg pseudoephedrine sustained release tablets were as follows: T max = 8.58 ± 2.47 h; C max = 358.47 ± 140.43 mg / ml; ) = 10.27 ± 3.04 h; MRT = 18.84 ± 4.12 h; AUC = 7403.6 ± 2166.6 ng · h / ml.