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目的研究α硫辛酸可否在高盐诱导的高血压的发生发展中通过降低延髓头端腹外侧(RVLM)线粒体中活性氧(ROS)的过量产生来缓解高血压反应。方法按照体重将雄性大鼠随机分到2组:正常盐饮食(0.3%NaCl)8周和高盐饮食8周(以8%NaCl诱导高血压模型)(均n=14)。分别再分2个亚组:实验组(高血压模型+α硫辛酸)、模型组(高血压模型+0.9%NaCl)、对照组(正常盐饮食+α硫辛酸)和空白对照组(正常盐饮食+0.9%NaCl,均n=7)。在实验结束时,监测各组大鼠动脉压的变化;用ELISA测定丙二醛(MDA)、超氧化物歧化酶(SOD)以及谷胱甘肽(GSH)等含量或活性。用高效液相色谱法检测血清去甲肾上腺素(NE)水平。结果给与α硫辛酸9周后,模型组与实验组的平均动脉压(MAP)分别为(160.2±10.9),(128.2±9.6)mm Hg,差异有统计学意义(P<0.05)。模型组与实验组的MDA分别为(6.1±3.0),(9.8±3.2)μmol·g~(-1),实验组明显降低,差异有统计学意义(P<0.05);这2组的SOD水平分别为(16.2±1.3),(31.9±2.8)U·mg~(-1);这2组的GSH分别为(2.0±0.4),(4.9±0.8)μmol·g~(-1),实验组均明显增高,差异均有统计学意义(均P<0.05)。模型组与实验组的血清去甲肾上腺素分别为(458.9±6.1),(322.8±7.2)pg·mL~(-1),差异有统计学意义(P<0.05)。结论长期补充α硫辛酸可通过提高抗氧化能力来缓解高血压反应。
Objective To investigate whether α-lipoic acid can relieve the hypotensive response by decreasing the excessive production of reactive oxygen species (ROS) in the mitochondria of the rostral ventrolateral medulla (RVLM) during the development of high salt-induced hypertension. Methods Male rats were randomly divided into 2 groups according to body weight: normal salt diet (0.3% NaCl) for 8 weeks and high salt diet for 8 weeks (8% NaCl induced hypertension model) (n = 14). The rats were divided into two subgroups: experimental group (model of hypertension + alpha lipoic acid), model group (model of hypertension + 0.9% NaCl), control group (normal salt diet + alpha lipoic acid) and blank control group Diet + 0.9% NaCl, all n = 7). At the end of the experiment, the changes of arterial pressure in each group were monitored. The content and activity of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH) were measured by ELISA. Serum norepinephrine (NE) levels were measured by high performance liquid chromatography. Results The mean arterial pressure (MAP) of the model group and the experimental group were (160.2 ± 10.9) and (128.2 ± 9.6) mm Hg, respectively, after 9 weeks of administration of α-lipoic acid. The difference was statistically significant (P <0.05). The MDA in the model group and the experimental group were (6.1 ± 3.0) and (9.8 ± 3.2) μmol · g -1, respectively, and the difference was statistically significant (P <0.05). The SOD (16.2 ± 1.3) and (31.9 ± 2.8) U · mg ~ (-1), respectively. The GSH in these two groups were (2.0 ± 0.4) and (4.9 ± 0.8) μmol · g -1 The experimental group were significantly increased, the differences were statistically significant (P <0.05). The levels of norepinephrine in model group and experimental group were (458.9 ± 6.1) and (322.8 ± 7.2) pg · mL -1, respectively, with statistical significance (P <0.05). Conclusion Long-term supplementation with alpha lipoic acid can relieve the hypotensive response by increasing the antioxidant capacity.