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理论上孕妇外周血中的胎儿细胞可做为新的标本来源,它可以替代羊膜穿刺术和绒毛膜活检所得到的样品。本文作者使用多聚酶链反应(polymerase chain reaction,PCR)检测孕妇静脉血中大量母体细胞中所含少量的胎儿细胞。PCR方可扩增外周血里DNA中一个Y-特异性重复序列。胎儿是男性的孕妇外周血DNA中的Y-特异性序列得以扩增,而胎儿是女性的则无扩增现象。作者认为这是首次利用孕妇血液进行分子遗传学分析的报道。
Theoretically fetal cells in the peripheral blood of pregnant women can be used as a new source of specimens, it can replace amniocentesis and chorionic villus samples obtained. The authors used polymerase chain reaction (PCR) to detect a small amount of fetal cells contained in a large number of maternal cells in pregnant women’s venous blood. PCR can amplify a Y-specific repeat in the DNA in the peripheral blood. The Y-specific sequence in the peripheral blood DNA of a pregnant woman whose fetus is a male is amplified, whereas the fetus is a female without amplification. The authors believe this is the first report using molecular genetic analysis of pregnant women’s blood.