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目的检测高浓度甲基苯丙胺(MA)对中脑边缘多巴胺神经元自发动作电位频率和超极化激活阳离子电流(Ih)的影响。方法应用立体定位仪将逆行示踪剂注入伏隔核区(NAc),标记投射到该区域的中脑边缘多巴胺神经元,并进行酪氨酸羟化酶(TH)染色鉴定;制备中脑脑片,应用膜片钳技术在全细胞模式下检测MA(10μmol/L)对标记神经元自发动作电位频率及Ih的作用。结果中脑边缘投射多巴胺神经元可被准确示踪标记,呈TH阳性,位于腹侧被盖区(VTA);MA可明显降低其自发动作电位频率(P<0.05),并抑制Ih电流(P<0.05)。结论甲基苯丙胺可抑制中脑边缘多巴胺神经元的自发动作电位频率和Ih电流。
Objective To investigate the effect of high concentration methamphetamine (MA) on the spontaneous action potential frequency and hyperpolarization-activated cation current (Ih) in the midbrain dopamine neurons. Methods The retrograde tracer was injected into the nucleus accumbens (NAc) by stereotactic apparatus and the dopaminergic neurons projecting to the midbrain edge were labeled and stained with tyrosine hydroxylase (TH) The patch-clamp technique was used to detect the effect of MA (10μmol / L) on the frequency of spontaneous action potentials and Ih in labeled neurons in whole-cell mode. Results The dopaminergic neurons projecting at the midbrain edge could be accurately labeled with TH and located in the ventral tegmental area (VTA). MA significantly decreased the frequency of spontaneous action potentials (P <0.05) and inhibited the Ih current (P <0.05). Conclusions Methamphetamine can inhibit the spontaneous action potential frequency and Ih current of dopaminergic neurons in the midbrain.