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AIM:To examine the effect of interleukin-l-beta (IL-1β)promoter region C-511T and IL-1 receptor antagonist(IL-1RN) polymorphism among the patients with chronichepatitis B virus (HBV) infection (HCC and non-HCC).METHODS:Genomic DNA from 136 Thai patients withchronic HBV infection (HCC=46 and non-HCC=90) and152 healthy individuals was genotyped for IL-1β genepolymorphism (-511) using polymerase chain reactionwith sequence specific primers (PCR-SSP).The variablenumber of tandem repeats (VNTR) of IL-1RN gene wasassessed by a PCR-based assay.The association betweenthese genes and status of the disease was evaluated byX~2 test.RESULTS:IL-1B-511 genotype C/C was found tobe significantly different in patients with HCC whencompared with healthy individuals (P=0.036,OR=2.29,95%CI=1.05-4.97) and patients without HCC (P=0.036,OR=2.52,95%CI=1.05-6.04).Analysis of allelefrequencies of IL-1B-511 showed that IL-1B-511 Callele was also significantly increased in patients withHCC,compared to that in healthy control (P=0.033,OR=1.72,95%CI=1.04-2.84).However,no significantassociation in IL-1RN gene was found between the twogroups.CONCLUSION:IL-1B-511C allele,which may beassociated with high IL-1B production in the liver,is agenetic marker for the development of HCC in chronic hepatitis B patients in Thai population.
AIM: To examine the effect of interleukin-1-beta promoter region C-511T and IL-1 receptor antagonist (IL-1RN) polymorphism among the patients with chronic hepatitis B virus (HBV) infection HCC) .METHODS: Genomic DNA from 136 Thai patients with chronic HBV infection (HCC = 46 and non-HCC = 90) and 152 healthy individuals was genotyped for IL-1β genepolymorphism (-511) using polymerase chain reaction with sequence specific primers ). The variable number of tandem repeats (VNTR) of IL-1RN gene was identified by a PCR-based assay. The association betweenthese genes and status of the disease was evaluated by X ~ 2 test. RESULTS: IL-1B-511 genotype C / C was found to significantly differ in patients with HCC whencompared with healthy individuals (P = 0.036, OR = 2.29, 95% CI = 1.05-4.97) and patients without HCC (P = 0.036, OR = 2.52, 95% CI = 1.05-6.04 ). Analysis of allele sequences of IL-1B-511 showed that IL-1B-511 Callele was also significantly increased in patients with HCC, compared to that in h no significant association in IL-1RN gene was found between the twogroups.CONCLUSION: IL-1B-511C allele, which may be associated with high IL (P = 0.033, OR = 1.72, 95% CI = 1.04-2.84) -1B production in the liver, is an age marker for the development of HCC in chronic hepatitis B patients in Thai population.