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对已克隆化分离的一个β地贫基因,应用6种人工合成的寡核苷酸为内引物。以[α-~(35)S]dATP进行标记,用Sanger双脱氧末端终止法进行序列测定。结果表明,此例β珠蛋白基因的密码子41/42中缺失了4个核苷酸(TCTT)。
For a β thaliana gene that has been cloned and isolated, six synthetic oligonucleotides were used as internal primers. The [? ~ (35) S] dATP was used for the sequence determination by Sanger dideoxy terminator method. The results showed that the codon 41/42 of this β-globin gene was missing 4 nucleotides (TCTT).