论文部分内容阅读
目的研究外源性硫化氢对氧化型低密度脂蛋白(ox-LDL)诱导内皮细胞组织因子(TF)和组织因子途径抑制物(TFPI)表达的影响。方法培养人脐静脉内皮细胞(HUVEC),分别以不同浓度NaHS(25、50、100及200μmol/L)和50 mg/L ox-LDL共孵育,RT-PCR和ELISA分别检测TF和TFPI mRNA表达和蛋白含量。结果用50mg/L ox-LDL孵育HUVEC 24 h后,TF mRNA表达上调8倍,蛋白含量增加7倍(P<0.01);而TFPI mRNA表达降低73%,蛋白含量减少65%(P<0.01)。用25、50、100及200μmol/L NaHS预孵育HUVEC 1 h,再与ox-LDL共同孵育,与ox-LDL组相比,加入不同浓度NaHS组TF mRNA分别降低12%、31%、63%和80%(P<0.05),蛋白含量分别降低13%、30%、62%和77%;TFPI mRNA表达分别升高0.6、1.2、2.0和2.6倍,蛋白含量分别升高0.3、0.7、1.1和1.6倍。结论 NaHS能抑制ox-LDL对内皮细胞TF表达的诱导作用,减轻ox-LDL对内皮细胞TFPI表达的抑制作用。
Objective To investigate the effect of exogenous hydrogen sulfide on the expression of tissue factor (TF) and tissue factor pathway inhibitor (TFPI) in endothelial cells induced by ox-LDL. Methods Human umbilical vein endothelial cells (HUVECs) were cultured and incubated with different concentrations of NaHS (25, 50, 100 and 200 μmol / L) and 50 mg / L ox-LDL, respectively. The mRNA expressions of TF and TFPI were detected by RT- And protein content. Results HUVEC incubated with 50 mg / L ox-LDL for 24 h up-regulated the mRNA expression of TF by 8-fold and increased the protein content by 7-fold (P <0.01), while the expression of TFPI mRNA decreased by 73% . HUVECs were preincubated with 25, 50, 100 and 200 μmol / L NaHS for 1 h and then incubated with ox-LDL. Compared with the ox-LDL group, TF mRNA decreased by 12%, 31% and 63% And 80% respectively (P <0.05), and the protein content decreased by 13%, 30%, 62% and 77% respectively. The expression of TFPI mRNA increased by 0.6,1.2,2.0 and 2.6 times respectively, and the protein content increased by 0.3,0.7,1.1 And 1.6 times. Conclusion NaHS can inhibit the induction of TF expression in endothelial cells by ox-LDL and reduce the inhibitory effect of ox-LDL on the expression of TFPI in endothelial cells.