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1981年DeBold第一次用生理盐水和pH7.2的磷酸盐缓冲溶液提取出了大鼠心房肌中的心钠素。1984年一些实验室在纯化心钠素时多用1mol/L醋酸溶液提取,国内也沿用这种方法从动物组织中提取心钠素。用这一方法取得心钠素的提取物,需要用低温高速离心机及冰
In 1981, DeBold extracted atrial natriuretic peptide from rat atrial muscle for the first time using physiological saline and pH 7.2 phosphate buffer solution. In 1984, some laboratories used 1 mol/L acetic acid solution for the purification of atrial natriuretic peptides. This method is also used in China to extract atrial natriuretic peptides from animal tissues. Using this method to obtain an atrial natriuretic extract requires cryogenic high-speed centrifuges and ice