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本研究根据形态学和ITS序列分析结果,将一株分离自海南东寨港红树林保护区红海榄根际土壤的菌株XGH2321鉴定为青霉属的一种真菌。通过对培养基中的碳源、氮源和盐度的优化,确定了适合该真菌菌株分泌抑菌活性物质的改良查氏培养基(4%玉米浆,0.3%NaNO3,0.05%KCl,0.1%K2HPO4,0.05%MgSO4,pH 7.4,9%盐度)。将活化后的菌株XGH2321接种到该培养基中,按28°C、160 r/min振荡培养7 d,获得的发酵液水萃取物对金黄色葡萄球菌、藤黄八叠球菌和枯草芽孢杆菌的生长具有明显的抑制作用(MIC为400μg/mL),而发酵液的乙酸乙酯萃取物对上述微生物的生长也具有一定的抑制作用(MIC为800μg/mL);同时,上述2种萃取物对植物病原菌立枯丝核菌的生长也具有明显的抑制作用,MIC分别为200μg/mL和400μg/mL。
In this study, based on morphological and ITS sequence analysis results, a strain isolated from rhizosphere soil of Rhizophora stylosa in Mangrove Reserve of Dongzhaigang in Hainan Province was identified as a fungus of Penicillium. By optimizing the carbon source, nitrogen source and salinity in the medium, the modified Charcter culture medium (4% corn steep liquor, 0.3% NaNO3, 0.05% KCl, 0.1% K2HPO4, 0.05% MgSO4, pH 7.4, 9% salinity). The activated strain XGH2321 was inoculated into the medium and cultured at 160 r / min for 28 days with shaking for 7 days. The obtained aqueous extract of the fermentation broth was stable against Staphylococcus aureus, Sarcina lutea and Bacillus subtilis (MIC 400μg / mL), while the ethyl acetate extract of fermentation broth also inhibited the growth of the above microorganisms (MIC 800μg / mL). Meanwhile, the above two extracts The growth of plant pathogen Rhizoctonia solani was also significantly inhibited with MICs of 200 μg / mL and 400 μg / mL, respectively.