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以蕌头鳞茎盘(basilar tray of bulbs)为外植体,在 1/2MS+BA1.0mg/L+IBA0.2mg/L培养基上、温度23—25℃条件下试管培养。结果显示:诱导分化、增殖及生根一步完成, 获得完整植株,36d一个培养周期,72d增殖倍数为128,以普通沙土为移栽基质,移栽成活率100%。
The basilar tray of bulbs was used as explants and cultured in vitro on 1 / 2MS + BA1.0mg / L + IBA0.2mg / L medium at 23-25 ℃. The results showed that the induction of differentiation, proliferation and rooting were completed in one step. The whole plant was obtained and cultured for 36 days. The multiplication rate at 72d was 128. The average survival rate of transplanting was 100%.