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目的研究白血病细胞株HL-60中基质金属蛋白酶2(MMP-2)和基质金属蛋白酶天然抑制剂-2(TIMP-2)的表达及细胞因子干扰素(IFN-γ)对MMP-2和TIMP-2转录水平的调节。方法分别在HL-60细胞生长的不同时期(1~5d)收集细胞,采用半定量RT-PCR方法,分析白血病细胞株HL-60中MMP-2和TIMP-2 mRNA的表达水平;以不同浓度的IFN-γ作用于HL-60细胞,24、48h后RT-PCR检测MMP-2和TIMP-2mRNA水平的变化。结果细胞接种后24h MMP-2的mRNA水平最高。IFN-γ各个浓度组对MMP-2 mRNA表达均有抑制作用(P<0.05),且随着作用浓度的增高mRNA表达减少;能够增强TIMP-2mRNA表达(P<0.05)。结论明确了白血病细胞株HL-60株在转录水平表达MMP-2和TIMP-2;IFN-γ对MMP-2 mRNA水平有明显的抑制作用,而对TIMP-1mRNA水平有增强作用。
Objective To investigate the expression of matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase-2 (TIMP-2) and the effect of interferon-γ on the expression of MMP-2 and TIMP in leukemia cell line HL- -2 transcription level regulation. Methods The cells were collected in different periods (1-5 days) of HL-60 cells. The expression of MMP-2 and TIMP-2 mRNA in leukemia cell line HL-60 was analyzed by semi-quantitative RT-PCR. IFN-γ in HL-60 cells. The changes of MMP-2 and TIMP-2 mRNA were detected by RT-PCR 24 and 48 hours after treatment. Results The mRNA level of MMP-2 was the highest at 24h after inoculation. The levels of MMP-2 mRNA in IFN-γ groups were significantly decreased (P <0.05). The mRNA expression of TIMP-2 was increased with the concentration of IFN-γ increased (P <0.05). Conclusions It is clear that leukemia cell line HL-60 expressed MMP-2 and TIMP-2 at transcription level. IFN-γ significantly inhibited MMP-2 mRNA level and enhanced TIMP-1 mRNA level.