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通过地高辛标记的Flammutoxin基因特异性探针与金针菇(Flammulina velutipes)菌丝体、菌柄和菌盖中的mRNA进行杂交,结果表明在金针菇的菌柄中有该蛋白质的表达,而在菌丝体和菌盖中均无该蛋白质的表达.此外在检测的平菇(Pleurotus ostreatus)、香菇(Lentinus edodes)、杏鲍菇(P.eryngii)和双孢蘑菇(Agaricus bisporus)中也无该蛋白质基因的表达.
The digoxigenin labeled Flammutoxin gene-specific probe hybridized with the mRNA in the mycelium, stipe and cap of Flammulina velutipes. The results showed that the protein was expressed in the stipe of Flammulina velutipes, There was no expression of this protein in the filament or cap.Furthermore, no detectable Pleurotus ostreatus, Lentinus edodes, P.eryngii and Agaricus bisporus were detected Protein gene expression.