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目的观察多器官功能障碍综合征(MODS)大鼠小肠胆碱能/氮能神经-Cajal 间质细胞(ICC)网络形态学的变化并探讨其意义。方法 Wistar 大鼠随机分为对照组(20只)、MODS 模型组(20只),行小肠肌层全厚标本 c-Kit 和乙酰胆碱囊泡状转运体(VAChT)/神经性一氧化氮合酶(nNOS)免疫荧光双重标记,共聚焦显微镜观测小肠神经-ICC 网络结构的变化并进行定量分析。结果对照组单个高倍视野 ICC 和胆碱能/氮能神经纤维数量分别为:(18.0±2.2)个,(28.0±1.3)个,(16.0±0.3)个;c-Kit 和 VAChT/nNOS 免疫阳性荧光强度分别为:(30.0±3.4)、(208.0±10.4)、(222.0±4.4)U;MODS 模型组 ICC 和胆碱能/氮能神经纤维数量分别为:(6.0±0.5)个,(13.0±1.1)个,(7.0±0.5)个;c-Kit 和 VAChT/nNOS 免疫阳性荧光强度分别为:(8.0±1.2)、(98.0±9.1)、(96.0±7.9)U;对照组各组数据与 MODS 模型组相应的数据比较有明显的差异(P<0.01),MODS 组小肠神经-ICC 网络结构不连续。结论 MODS 大鼠小肠胆碱能/氮能神经-ICC 网络结构受到明显破坏,MODS 大鼠胃肠运动障碍可能与小肠神经-ICC 网络损伤有关。
Objective To observe the morphological changes of small intestine cholinergic / aorta-Cajal interstitium (ICC) in multiple organ dysfunction syndrome (MODS) rats and its significance. Methods Wistar rats were randomly divided into control group (n = 20), MODS model group (n = 20), c-Kit and VAChT / neuronal nitric oxide synthase (nNOS) immunofluorescence double labeling, confocal microscopy changes in small intestine nerve-ICC network structure and quantitative analysis. Results The number of single high power field ICC and cholinergic / Nerve fibers in the control group were (18.0 ± 2.2), (28.0 ± 1.3) and (16.0 ± 0.3), respectively. The positive immunoreactivity of c-Kit and VAChT / nNOS (30.0 ± 3.4), (208.0 ± 10.4) and (222.0 ± 4.4) U, respectively. The numbers of ICC and cholinergic / anelgic nerve fibers in MODS model group were (6.0 ± 0.5) and ± 7.0), respectively. The immunofluorescence intensity of c-Kit and VAChT / nNOS were (8.0 ± 1.2), (98.0 ± 9.1) and (96.0 ± 7.9) U, respectively Compared with MODS model group, there was significant difference (P <0.01). The structure of small intestine neural-ICC in MODS group was discontinuous. Conclusions The structure of small intestine cholinergic / nitric oxide-ICC network in MODS rats is obviously damaged. The gastrointestinal motility disorder in MODS rats may be related to the damage of intestinal nerve-ICC network.