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[Objective] To extract and isolate tigogenin from Agave sisalana Perrine.[Method] The tigogenin was extracted by heating reflux method and purified by macroporous resin,and the optimum technology was determined.[Results] D-101 macroporous resin was endowed with better purification effect and the optimum purification conditions were as follows:70% alcohol as eluent,eluent dosage of 100 ml,elution velocity of 0.6 ml/min.Under the above conditions,the recovery rate and purity of tigogenin were 79.1% and 71.6% respectively.[Conclusion] The method adopted in this study was feasible for industrial production of tigogenin.
[Objective] To extract and isolate tigogenin from Agave sisalana Perrine. [Method] The tigogenin was extracted by heating reflux method and purified by macroporous resin, and the optimum technology was determined. [Results] D-101 macroporous resin was endowed with better purification effect and the optimum purification conditions were as follows: 70% alcohol as eluent, eluent dosage of 100 ml, elution velocity of 0.6 ml / min.Under the above conditions, the recovery rate and purity of tigogenin were 79.1% and 71.6% respectively. [Conclusion] The method adopted in this study was feasible for industrial production of tigogenin.