论文部分内容阅读
为了探讨马尾松Pinus massoniana针叶营养物质含量与其对松突圆蚧Hemiberlesia pitysophila Takagi抗性的关系,选择了不同抗性的10个马尾松家系,分别测定未受松突圆蚧危害植株与受害植株针叶中可溶性糖、总糖、可溶性蛋白、全N、游离氨基酸、粗脂肪等营养物质的含量和总糖/全N等7个指标,并采用判别分析法分析这7个指标与马尾松家系对松突圆蚧抗性的关系。结果表明:马尾松家系不论是否受松突圆蚧的危害,能明显区别抗性组别的指标都是可溶性蛋白(X3)、全N(X4)和游离氨基酸总量(X7),随抗性的降低,它们的含量呈上升趋势,受害植株中上述3个指标的含量低于未受害植株;受害后,各指标变化量中能明显区别抗性组别的是全N(X4),随着植株抗性的降低,针叶中的可溶性蛋白(X3)、全N(X4)和游离氨基酸总量(X7)的含量变化量减小。据此得出:(1)可溶性蛋白(X3)、全N(X4)和游离氨基酸总量(X7)是与抗性关系最密切的指标,含量与抗性呈负相关关系,变化量与抗性呈正相关关系;(2)受到危害后,无论含量或变化量,7个指标都发生了重要变化,都表现出与抗性的相关性,构成了有效判别函数的组成变量;(3)因判别分析方法可以分析各指标的综合作用,应用该方法分析各营养成分与马尾松对松突圆蚧抗性的关系,比用方差分析和多重比较方法更有优越性。上述研究结果为抗松突圆蚧马尾松家系的选育及其抗性机理的深入研究,提供了重要的科学参考价值。
In order to investigate the relationship between Pinus massoniana coniferous nutrient content and resistance to Hemiberlesia pitysophila Takagi, ten families of Pinus massoniana with different resistances were selected to test the effects of the disease- Seven indicators of soluble sugar, total sugar, soluble protein, total N, free amino acids, crude fat and other nutrients and the total sugar / total N in the needles. The seven indicators were analyzed with discriminant analysis The relationship between the resistance of Syringa pitysophila. The results showed that no significant difference was found in the susceptibility of Pinus massoniana families regardless of whether they were susceptible to P. crassiphylla. The indexes of all the resistant groups were soluble protein (X3), total N (X4) and total free amino acids (X7) The contents of these three indicators were lower than that of the unharmed plants. After the victim, the total N (X4) in the variation of each index was obviously different from that of the resistant group, and with the increase of The decrease of plant resistance decreased the content of soluble protein (X3), total N (X4) and total free amino acids (X7) in conifer. The results showed that: (1) Soluble protein (X3), total N (X4) and total free amino acids (X7) were the most closely related indicators of resistance and the content and resistance were negatively correlated. (2) After being harmed, no significant changes were made to all the 7 indicators, regardless of content or amount of change. All of them showed the correlation with resistance and constituted the constituent variables of effective discriminant function. (3) Discriminant analysis method can analyze the comprehensive effect of each index. By using this method, the relationship between each nutrient component and Masson pine seedling resistance was analyzed, which was more superior than ANOVA and multiple comparison methods. The above results provide an important scientific reference value for the breeding of Pinus massoniana and its resistance mechanism.