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目的探讨饮水氟暴露对成年男性性激素结合球蛋白(SHBG)及睾酮水平的影响。方法依据水氟检测结果,在河南省通许县随机选择3个村庄作为调查点,分别为高氟村、改水村和对照村;整群抽取调查区本地生长的18～50岁成年男性,收集空腹静脉血和晨尿。应用氟离子选择电极法测定饮用水和尿中氟的含量;用酶联免疫吸附法(ELISA)测定其血清中SHBG水平,化学发光法测定血清睾酮的含量。结果血清SHBG浓度分别为对照组47.85nmol/L,改水组31.37nmol/L,高氟组24.52nmol/L,3组比较差异均有显著性(P<0.05)。血清睾酮水平分别为对照组3.69ng/ml,改水组4.61ng/ml,高氟组4.83ng/ml,高氟与对照组比较,差异有显著性(P<0.05)。高氟组血清SHBG水平与睾酮呈正相关关系(r=0.230,P=0.049),而在改水组和对照组中未观察到相关关系。结论长期氟暴露可影响成年男性血清SHBG水平和睾酮水平,并可能存在剂量-效应关系。
Objective To investigate the effects of drinking fluoride exposure on sex hormone binding globulin (SHBG) and testosterone levels in adult male. Methods Based on the results of water fluoride test, three villages were randomly selected from Tongxu County of Henan Province as high fluoride, water and control villages; Venous blood and morning urine. Fluoride ion-selective electrode method was used to determine the content of fluoride in drinking water and urine. SHBG level in serum was determined by enzyme-linked immunosorbent assay (ELISA), and serum testosterone was determined by chemiluminescence. Results Serum SHBG concentration was 47.85nmol / L in control group, 31.37nmol / L in water modification group and 24.52nmol / L in high fluoride group, respectively. There was significant difference between the three groups (P <0.05). Serum testosterone levels were 3.69ng / ml in control group, 4.61ng / ml in water modification group and 4.83ng / ml in high fluoride group, respectively. There was significant difference between high fluoride and control group (P <0.05). There was a positive correlation between serum SHBG levels and testosterone in high fluoride group (r = 0.230, P = 0.049), but no correlation was found between water-amended group and control group. Conclusion Long-term fluoride exposure may affect serum SHBG levels and testosterone levels in adult males and may have a dose-response relationship.