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目的:优选吐鲁番锦鸡儿中总黄酮提取纯化工艺条件。方法:以吐鲁番锦鸡儿根总黄酮含量为指标,采用正交试验设计法优选吐鲁番锦鸡儿根总黄酮提取的工艺条件;利用静态吸附方法筛选分离吐鲁番锦鸡儿根总黄酮的最适大孔树脂,利用动态吸附方法研究最合适大孔树脂纯化吐鲁番锦鸡儿总黄酮的条件,并用紫外分光光度法测定其含量。结果:最佳提取工艺条件为乙醇回流提取法,80%乙醇,料液比为1∶30,提取1 h,温度为80℃。最佳纯化工艺条件为上样液质量浓度5 mg·m L-1,上样液用量5 BV,上样液流速2 BV·h-1,洗脱剂浓度90%乙醇,洗脱剂流速2 BV·h-1,洗脱剂用量6 BV。结论:优选的工艺稳定、合理,可用于吐鲁番锦鸡儿根中总黄酮的提取及纯化。
Objective: To optimize the extraction and purification of total flavonoids from Caragana radish. Methods: According to the content of total flavonoids in Caragana microphylla Turcz as the index, orthogonal experimental design method was used to optimize the extraction conditions of total flavonoids in Caragana microphylla Turcz. The optimum macroporous resin was isolated by static adsorption method, The optimum conditions for the purification of total flavonoids from turmeric Carassius auratus by macroporous resin were studied by dynamic adsorption method. The content of total flavonoids was determined by UV spectrophotometry. Results: The optimum extraction conditions were ethanol reflux extraction, 80% ethanol, the ratio of material to liquid was 1:30, extraction time was 1 h and the temperature was 80 ℃. The best purification process conditions were as follows: the mass concentration of sample solution was 5 mg · mL-1, the amount of sample solution was 5 BV, the sample flow rate was 2 BV · h-1, the eluent concentration was 90% ethanol and the eluent flow rate was 2 BV · h-1, the amount of eluent 6 BV. Conclusion: The optimized process is stable and reasonable. It can be used for extraction and purification of total flavonoids from Caragana microphylla Turcz.