目的评价纳米刀消融技术应用于活体猪肺组织的有效性与安全性。方法取健康巴马小型猪5只(实验动物A、B、C、D、E)用于实验,于CT引导下经皮穿刺纳米刀消融猪肺组织,分别于消融后1天、2周、1个月、2个月、4个月随机抽取1只行肺部CT扫描后处死实验动物,整体切除右肺并制作病理切片进行组织学观察。结果对所有实验动物均顺利完成消融。实验动物E、F出现少许气胸,余未见并发症。CT图像示消融区与周围组织分界较清晰,内部支气管、血管管腔形态完整。消融后1天消融区呈磨玻璃密度;消融后2周消融区密度不均匀增高,范围减小;消融后1个月及2个月高密度范围增大且密度较前减低;消融后4个月消融区范围减小,密度较均匀。各时间点病理切片均可见不同程度肺水肿及炎性浸润。术后1天、2周炎细胞浸润及肺水肿明显,1个月、2个月及4个月可见大量纤维增生及明显再生修复。结论应用纳米刀技术对猪肺组织进行消融安全、有效。 Objective To evaluate the effectiveness and safety of nano-knife ablation in living pig lung tissues. Methods Five healthy Bama miniature pigs (experimental animals A, B, C, D and E) were used in the experiment. The lung tissues of pigs were ablated by CT guided percutaneous puncture. The animals were sacrificed at 1, One month, two months and four months were randomly selected 1 lung CT scan after sacrifice the experimental animals, the whole right lung and pathological sections were made for histological observation. Results All the experimental animals were successfully completed ablation. Experimental animals E, F a little pneumothorax, I did not see complications. CT image shows the boundary between the ablation zone and the surrounding tissue more clearly, the internal bronchus, vascular lumen integrity. At 1 day after ablation, the ablation area showed the density of ground glass; after 2 weeks of ablation, the density of ablation area increased unevenly and the range was reduced; the high density range at 1 month and 2 months after ablation increased and the density was lower than before; Month ablation area reduced, the density is more uniform. Pathological sections at all times showed varying degrees of pulmonary edema and inflammatory infiltration. One day after operation, inflammatory cell infiltration and pulmonary edema were obvious at 2 weeks. A large number of fibrous hyperplasia and obvious regenerative repair were observed at 1 month, 2 months and 4 months. Conclusion It is safe and effective to ablate pig lung tissue with nano-knife technique.