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为了研究黄瓜花叶病毒(CMV)诱导的岷江百合(Lilium regale)病程相关蛋白PR10基因在抗病毒防御反应中的作用,对岷江百合叶片接种CMV,采用RACE技术获得岷江百合LrPR10的全长cDNA序列,并对其进行生物信息学分析;利用实时荧光定量PCR对LrPR10在各器官特异性以及CMV和水杨酸(SA)处理后的表达模式进行了测定分析。结果表明:LrPR10全长756 bp,可编码由157个氨基酸组成的蛋白质,该蛋白具有病程相关蛋白典型的Bet_v1_like保守结构域;LrPR10在岷江百合鳞茎中相对表达量最高,在嫩叶中最低;该基因可以被CMV和SA诱导上调表达,岷江百合、卷丹(L.lancifolium)和宜昌百合(L.leucanthum)在CMV接种处理后LrPR10的相对表达量分别在4 d、4 d和1 d达到最大值,分别为处理前的58倍、27倍和292倍,在SA处理后LrPR10的相对表达量都在8 h达到最大值,分别为处理前的34倍、8倍和38倍。以上结果表明LrPR10在岷江百合抗黄瓜花叶病毒防御反应过程中发挥作用。
In order to investigate the role of PR10 gene in cucumber mosaic virus (CMV) -induced disease-related protein PR10 in the anti-virus defense response of Minjiang Lily (Lilium regale), CMV was inoculated on the leaves of Minjiang lily and the full-length cDNA sequence of LrPR10 , And analyzed by bioinformatics. The expression patterns of LrPR10 in various organ-specific, CMV and salicylic acid (SA) treatments were determined by real-time fluorescence quantitative PCR. The results showed that: LrPR10 was 756 bp in length encoding a protein consisting of 157 amino acids with typical Bet_v1_like conserved domain of disease-related protein. LrPR10 had the highest relative expression in bulb and lowest in young leaves The expression of LrPR10 gene was up-regulated by CMV and SA, and the relative expression of LrPR10 mRNA in L. lily, L. lancifolium and L. leucanthum reached the maximum at 4 d, 4 d and 1 d, respectively Respectively. The relative expression levels of LrPR10 reached the maximum at 8 h after SA treatment, which were 34 times, 8 times and 38 times before treatment, respectively. The above results show that LrPR10 plays a role in defense against cucumber mosaic virus in Minjiang Lily.