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取3529×Oro(F_1)及其亲本的新鲜叶片,经组织捣碎、离心、得线粒体;用SDS破线粒体,过氯酸钠、氯仿除蛋白。透析纯化后,用核糖核酸酶除线粒体R NAS;再经Sepharose4 B柱层析得纯净的线粒体DNA(mitDNA)。电镜观察制备的mitDNA为共价闭合环状分子。3529、3529×Oro(F_1)、Oro的mitDNA的Tm值分别为85.3℃、85.0℃、86.0℃;增色值分别为23％、29％、5％;(G+C)％含量分别为39、38、41。杂种mitDNA与亲本的差异或接近也反映在其性状1上。如3529、杂种(F_1)、Oro的株高(cm)分别为163.1、173.2、411.8;全株有效果数分别为323.7、407.0、340.5;每果籽粒数分别为13.1、14.6、11.4;千粒重(g)分别为5.20、4.47、3.60;含油量(％)分别为38.75、42.60、37.10;单株产量(g)分别为14.2、18.7、12.4;亩产量(kg)分别为87、157、76,都优于双亲而更接近于母本3529。
Take 3529 × Oro (F_1) and its parents of fresh leaves, the organization smashed, centrifuged to obtain mitochondria; break with mitochondria SDS, sodium chlorate, chloroform in addition to protein. After dialyzing and purification, mitochondrial R NAS was removed by ribonuclease; pure mitochondrial DNA (mitDNA) was purified by Sepharose 4B column. The mitDNA prepared by electron microscopy was a covalently closed circular molecule. 3529,3529 × Oro (F_1). The mitochondrial DNA (mt) values of Oro were 85.3 ℃, 85.0 ℃ and 86.0 ℃, respectively. The values of colorimetric values were 23%, 29% and 5% 38,41. The difference or proximity of the hybrid mitDNA to the parent is also reflected in its trait1. The plant height (cm) of 3529, F_1 and Oro were 163.1, 173.2 and 411.8, respectively. The effective numbers of the whole plant were 323.7, 407.0 and 340.5, respectively. The number of seeds per plant was 13.1, 14.6 and 11.4, respectively. g) were 5.20,4.47,3.60 respectively; the oil content (%) was 38.75,42.60 and 37.10 respectively; the yield per plant (g) was 14.2, 18.7 and 12.4 respectively; the yield per mu (kg) Better than the parents and closer to the mother 3529.