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在单因素试验的基础上,采用Central Composite Design设计对秀珍菇多糖超声波协同微波提取工艺中的液料比、提取时间和微波功率3因子的最优化组合进行了定量研究,建立并分析了各因子与得率关系的数学模型。同时研究了秀珍菇多糖对DPPH的清除效果。结果表明:最佳的工艺条件为液料比10.5∶1(mL/g)、提取时间123 s和微波功率335 W。经试验验证,在此条件下,得率为26.95%,与理论计算值26.83%基本一致。说明回归模型能较好地预测秀珍菇中多糖的得率。秀珍菇多糖对DPPH自由基和羟基自由基都有一定的清除作用,分别在质量浓度50.34μg/mL~503.42μg/mL和12.08μg/mL~96.66μg/mL的范围内,其清除DPPH自由基和羟基自由基的能力分别为30.54%~51.40%和37.16%~95.87%,且清除效果与多糖质量浓度之间都存在明显的量效关系。
On the basis of single factor experiment, Central Composite Design was used to quantitatively study the optimal combination of liquid to material ratio, extraction time and microwave power in the ultrasonic synergistic microwave extraction of Pleurotus externes polysaccharides, and to establish and analyze the factors Mathematic model of relationship with yield. At the same time, we studied the scavenging effect of XJ mushroom polysaccharide on DPPH. The results showed that the optimal process conditions were liquid - to - solid ratio 10.5:1 (mL / g), extraction time 123 s and microwave power 335 W. The experimental verification, in this condition, the yield was 26.95%, and the theoretical value of 26.83% basically the same. This shows that regression model can better predict the yield of polysaccharides in Pleurotus. Pleurotus ostreatus polysaccharides have some scavenging effects on DPPH radical and hydroxyl radical, and they were screened in the range of 50.34μg / mL ~ 503.42μg / mL and 12.08μg / mL ~ 96.66μg / mL respectively, which scavenged DPPH radical And hydroxyl radical were 30.54% ~ 51.40% and 37.16% ~ 95.87% respectively, and there was a significant dose-effect relationship between the removal efficiency and polysaccharide concentration.