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目的:探讨表皮生长因子受体(EG-FR)、蛋白激酶B(PKB/Akt)、Ras相关因子-1(Raf-1)mRNA在上皮性卵巢癌组织中表达的临床意义、相关性及对预后的影响。方法:转录聚合酶链反应(RT-PCR)技术检测14例正常卵巢组织、16例卵巢良性肿瘤组织及61例上皮性卵巢癌组织中EGFR、Akt和Raf-1 mRNA的表达,并分析它们与临床病理参数之间的关系及其相关性。结果:卵巢癌组织中EGFR、AKT和Raf-1阳性表达率分别为78.7%(48/61)、80.3%(49/61)和75.4%(46/61),显著高于正常卵巢及卵巢良性肿瘤组织,P<0.05;三者表达水平均与卵巢癌手术病理分期及淋巴结转移有关,P<0.05;EGFR、Akt表达水平与细胞学分级有关,P<0.05;EGFR与Raf-1及Akt与Raf-1之间的联系有统计学意义,P<0.05。结论:上皮性卵巢癌组织EGFR、Raf-1和Akt的过表达与卵巢癌的发生发展及侵袭有关,可能为多靶点联合治疗提供新方向。
Objective: To investigate the clinical significance of the expression of epidermal growth factor receptor (EG-FR), protein kinase B (PKB / Akt) and Ras-related factor-1 (Raf-1) mRNA in epithelial ovarian cancer The impact of prognosis. Methods: The expressions of EGFR, Akt and Raf-1 mRNA in 14 cases of normal ovarian tissue, 16 cases of ovarian benign tumor and 61 cases of epithelial ovarian cancer were detected by RT-PCR. The relationship between clinicopathological parameters and their correlation. Results: The positive rates of EGFR, AKT and Raf-1 in ovarian cancer were 78.7% (48/61), 80.3% (49/61) and 75.4% (46/61), respectively, which were significantly higher than those in normal ovary and benign ovary (P <0.05). The expression of EGFR, Akt was correlated with cytological grade, P <0.05. The expressions of EGFR, Raf-1 and Akt were significantly correlated with lymph node metastasis, The correlation between Raf-1 was statistically significant, P <0.05. Conclusion: The overexpression of EGFR, Raf-1 and Akt in epithelial ovarian cancer may be related to the development and invasion of ovarian cancer, which may provide a new direction for multi-target combination therapy.