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目的 观察氧自由基对线粒体的损伤以及牛磺酸的保护作用。方法 采用氧自由基发生系统FeSO4 /VitC损伤线粒体 ,分离的肝线粒体分为 3组 :对照组 (A组 ) ,损伤组 (B组 ) ,牛磺酸保护组 (C组 )。分别测定线粒体呼吸功能、3种氧化酶活性、丙二醛含量。结果 B组线粒体状态 3速率、呼吸控制率、磷 /氧比、氧化磷酸化效率降低 ,说明线粒体呼吸功能和氧化磷酸化功能受损 ;B组线粒体电子传递链的琥珀酸氧化酶、NADH氧化酶、细胞色素氧化酶活性下降 ,说明氧自由基可损伤线粒体电子传递链 ;并且氧自由基可致线粒体丙二醛水平明显升高 ,造成线粒体脂质过氧化损伤。上述变化B组与A组、C组比较差异均有显著性 (P <0 0 5或P<0 0 1) ;C组与A组比较差异均无显著性。结论 牛磺酸有自由基清除作用和直接膜保护作用 ,可预防氧自由基对线粒体的损伤 ,对线粒体结构和功能起保护作用。
Objective To observe the damage of mitochondria by oxygen free radicals and the protective effect of taurine. Methods Mitochondria were injured by the FeSO 4 / VitC system. The isolated mitochondria were divided into three groups: control group (group A), injury group (group B) and taurine protection group (group C). Mitochondrial respiratory function, three kinds of oxidase activity and malondialdehyde were measured respectively. Results The mitochondrial state 3 rate, respiratory control rate, P / O ratio and oxidative phosphorylation efficiency decreased in group B, indicating that mitochondrial respiratory function and oxidative phosphorylation function were impaired. In group B, the mitochondrial electron transport chain succinate oxidase, NADH oxidase , Cytochrome oxidase activity decreased, indicating that oxygen free radicals can damage the mitochondrial electron transport chain; and oxygen free radicals can cause mitochondrial malondialdehyde levels were significantly increased, resulting in mitochondrial lipid peroxidation damage. There was significant difference between group B and group A and group C (P <0.05 or P <0.01). There was no significant difference between group C and group A. Conclusion Taurine has the function of free radical scavenging and direct membrane protection, which can prevent the damage of mitochondria by oxygen free radicals and protect the structure and function of mitochondria.