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我们应用Chamber和Boyde等人的方法并加以改良，建立了大鼠破骨细胞体外培养方法，并对培养破骨细胞进行了形态、骨吸收陷窝的观察和酸性磷酸酶（ACP）、抗酒石酸酸性磷酸酶（TRAP）及抗酒石酸酸性三磷酸腺苷酶（TrAPas）染色等方面的鉴定，结果均符合破骨细胞的特点［1］。由
We applied the method of Chamber and Boyde et al. And improved the method of culturing rat osteoclast in vitro. We observed the morphological and osteoclastic lacunae of cultured osteoclasts and observed the relationship between acid phosphatase (ACP), tartaric acid Acid phosphatase (TRAP) and acid tartrate-resistant adenosine triphosphatase (TrAPas) staining and other aspects of the identification, the results are in line with the characteristics of osteoclasts [1]. by