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自从Enders等开始应用组织培养方法检出和培养脊髓灰质炎病毒以后,许多重要的病毒性疾病的病原都用这一方法分离了出来。对这些疾病的致病原因就有了清楚的了解,并对其中有些疾病发现了有效的疫苗。传染性肝炎(甲型肝炎)和血清性肝炎(乙型肝炎)估计是由病毒引起的,但至今尚无体外测定的方法。肝炎抗原 Blumberg等首先用琼脂凝胶沉淀素的方法发现一澳大利亚土著的血清中有一种抗原,能与多次输血的血友病病人的抗血清起反应。
Since Enders et al. Began to detect and develop poliovirus using tissue culture methods, many of the major viral disease pathogens have been isolated in this way. The causes of these diseases are clearly understood and effective vaccines are found for some of these diseases. Infectious hepatitis (Hepatitis A) and Serum Hepatitis (Hepatitis B) are estimated to be caused by viruses, but no in vitro method has so far been available. Hepatitis antigen Blumberg and other first with the method of agar gel precipitin found in an Australian indigenous serum has an antigen, with many transfused hemophilia patients antisera reaction.