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尾核内多巴胺递质可能具有对抗电针镇痛的作用。尾核内注射胆硷能受体阻断剂东茛菪硷可阻断电针的镇痛作用。本文系经慢性埋藏套管向家兔两侧尾核注射胆硷能(?)体激动剂,毛果云香硷,观察对尾核内多巴胺浓度升高时所致的对抗电针镇痛作用的影响。痛阈是以辐射热照射家兔鼻尖,引起甩头的反射时测定的。实验时先测定基础痛阈,然后向两侧尾核注射7.5μg/μl的多巴胺人工脑脊液溶液2μl,注后马上电针10分钟。电针5分钟后测痛。每隔5分钟测痛阈1遍,共6遍,然后分两组。一组向尾核内注射10μg/μl的毛果云香硷人工脑脊液溶液2μl。注完后,每5分钟测痛阈1遍共6遍。对照组未注药,同样观察30分钟。最后,经组织学鉴定,取两侧套管尖端均在尾核的实验例进行统计分析。结果尾核内注射多巴胺(共19例)的痛阈变化过程为电针开始5分钟和10分钟时,痛阈升至155％和146％,然后缓缓下降。30分钟后,实验组向尾核内注射毛果云香硷(10例),则痛阈不再下降,反而逐渐回升。对照组(9例)未注药,痛阈继续下降,30分钟后降至基础痛阈的80％。两组间差异非常显著(P<0.01)。结果提示,尾核内注射毛果云香硷有加强电针镇痛的效应,可使尾核内多巴胺浓度升高所引起的对抗电针镇痛效应翻转过来。所以,尾核内多巴胺浓度增高时对抗电针镇痛的效应,可能是通过抑制尾核内的胆硷能中间神经元的活动而实现的。
The dopamine transmitter in the caudate nucleus may have an anti-electroacupuncture analgesic effect. Intracoronary injection of cholesterinergic receptor blocker Dongqin can block the analgesic effect of electroacupuncture. In this paper, we injected cholesterinergic agonists and pilocarpine nevus to the caudal nucleus of rabbits by chronic burial cannula to observe the anti-electroacupuncture analgesic effect caused by the increase of dopamine concentration in caudate nucleus. Impact. The pain threshold was determined by radiating heat to the tip of the rabbit’s nose and causing a shake to reflect. In the experiment, the basic pain threshold was measured first, then 2 μl of 7.5 μg/μl of dopamine artificial cerebrospinal fluid solution was injected into the caudal nucleus of both sides, and electroacupuncture was performed immediately after the injection for 10 minutes. Electro-acupuncture measured pain 5 minutes later. The pain threshold was measured every 5 minutes for a total of 6 passes and then divided into two groups. One group injected 2 μl of a 10 μg/μl solution of podocarpum artificial cerebrospinal fluid into the caudate nucleus. After the injection, the pain threshold was measured every 6 minutes for a total of 6 passes. The control group did not inject drugs and was similarly observed for 30 minutes. Finally, the histological identification of the cuffed nucleus was used for statistical analysis. [Results] The change of pain threshold of dopamine injection in caudal nucleus (19 cases in total) was 5 minutes and 10 minutes after the beginning of electroacupuncture. The pain threshold increased to 155% and 146%, and then decreased gradually. Thirty minutes later, in the experimental group, M. fruticosa was injected into the caudate nucleus (10 cases), the pain threshold no longer decreased, but gradually increased. In the control group (n=9), the pain threshold continued to decrease after the drug was not injected, and it decreased to 80% of the basal pain threshold 30 minutes later. The difference between the two groups was very significant (P < 0.01). The results suggest that the injection of pilocarpine in the caudate nucleus enhances the effect of electroacupuncture analgesia, which can reverse the anti-electroacupuncture analgesic effect caused by the increase of dopamine concentration in the caudate nucleus. Therefore, the effect of increasing the concentration of dopamine in the caudate nucleus against electroacupuncture analgesia may be achieved by inhibiting the activity of the biliary interneurons in the caudate nucleus.