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以高抗品种东农９６７４ 为父本， 以高感品种东农８７－１０４ 为母本， 杂交得到Ｆ２ 代群体。该群体经人工接种灰斑病１号生理小种后， 分别从抗、感材料中取１５ 株的叶片提取ＤＮＡ组成抗、感池， 用８２０ 个１０ｍ ｅｒ随机引物进行ＲＡＰＤ多态性分析， 其中３个引物在抗、感池间出现稳定的多态性标记ＯＰＫ０３８４０， ＯＰＭ１７１７００， ＯＰＯ１０９５０， 并在Ｆ２ 代个体中表现出抗性与多态性标记协同分离的趋势， 这三个标记与抗性基因Ｒｆ１ 的连锁顺序为ＯＰＫ０３８４０－Ｒｆ１－ＯＰＭ１７１７００－ＯＰＯ１０９５０， 连锁距离分别为１０．４ｃＭ－Ｒｆ１－１３．８ｃＭ－２６．１ｃＭ。
Using high resistant variety Dongnong 9674 as the male parent and high susceptible variety Dongnong 87-104 as the female parent, the F2 generation population was crossed. After artificial inoculation of the gray spot No.1 physiological races, the population was extracted from the leaves of 15 strains of anti-susceptible materials to construct the anti-sense and sense-pool of DNA, and RAPD analysis with 820 10 m er random primers Three primers showed stable polymorphic markers OPK03840, OPM171700 and OPO10950 in the anti-sense and sense-sense pools, and displayed a synergistic separation of resistance and polymorphic markers in F2 progenies. These three markers were associated with resistance genes The linkage order of Rf1 is OPK03840-Rf1-OPM171700-OPO10950, with the linkage distances of 10.4cM-Rf1-13.8cM-26.1cM respectively.